k562 cell culture

/Type /FontDescriptor Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. H��Wێ��}�W��␔(QyH�ǆ7�z�� 3y��fx��|}��͋F� 6�[$��ԩS�w8ԓ��fB ��$X{�|�'��+?��؜&>&��6�dF� _�'��2�T�9�m޴��T�$��e�!�L]��xΊL7�Fs��"?��;��&}6�/�P�Ʌ�2��? 118: 355-361, 1977. Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. CCL-243) was grown in RPMI 1640 culture medium as detailed in the Supplementary Methods (online only, available at www.exphem. Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations. /FontName /ArialMT Biol. VI. PubMed: 8647842, Chan YJ, et al. The paired K562-s and K562-r clones are useful cell line models of sensitivity and resistance to a tyrosine kinase inhibitor used in the treatment of chronic myeloid leukemia. 59: 77-82, 1977. PubMed: 8557663, Tiffany HL, et al. PubMed: 7194480, Dimery IW, et al. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services. << Also Known As: Species: Human . Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. Cultures can be maintained by the addition or replacement of fresh medium. While we are not currently experiencing delays due to this pandemic, we expect that we could see them as the situation evolves. Cells were cultured in RPMI containing 10% fetal calf serum with addition of penicillin/streptomycin. | Chem. This study was designed to compare the effects of X-ray and γ-irradiation on K562 cells in natural killer (NK) cell expansion. /Type /Font Studies conducted by Anderson, et al., on the surface membrane properties led to the conclusion that the K-562 was a human erythroleukemia line. The stemline chromosome number is triploid with the 2S component occurring at 4.2%. /FontStretch /Normal /Font Can be used as a highly sensitive target for the in vitro natural killer assay. 10 0 obj Degradation of oxidized proteins in K562 human hematopoietic cells by proteasome. >> Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. PubMed: 95026, Andersson LC, et al. /StemV 88 9 0 obj K562--a human erythroleukemic cell line. PubMed: 163658, Lozzio BB, Lozzio CB. /Length 1996 The powders were dissolved in 80% ethanol and subsequently … 70: 5312-5328, 1996. /Type /ExtGState << PubMed: 8970984, Nauseel WM, et al. Homo sapiens, human endobj Ethical Standards for Obtaining Human Materials, Iscove's Modified Dulbecco's Medium (IMDM) (ATCC, Nucleic Acids, Proteins and Cell Extracts. J. Characteristics of expanded NK cells were identified by flow cytometry. /TT4 6 0 R K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic and monocytic series. PubMed: 7769849. /FontFamily (Times New Roman) PubMed: 3857109, Wu SQ, et al. The K562 subline K562r (resistant to butyrate) was purchased from DSM, Braunschweig, Germany (DSM ACC 10). /Flags 32 stream 30-2005. 1. Exp. K562 cells cultured in RMPI-1640 with 0.4% FBS were seeded in 12-well plates with 5 × 10 5 cells/well. org). Tissue: blood To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Occurrence of the Philadelphia chromosome, however, was of much lower frequency; none detected in 15 metaphases examined. The X was disomic, and N9 was nullisomic. An email will be sent to you with instructions. /op false /XHeight 515 Human myeloid leukemia cell lines: a review. Two distinct upstream regulatory domains containing multicopy cellular transcription factor binding sites provide basal repression and inducible enhancer characteristics to the immediate-early IES (US3) promoter from human cytomegalovirus. Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. /Ascent 905 << Don't see what you are looking for? %�� Leukemia 9: 858-862, 1995. 70: 8590-8605, 1996. Effect of the R569W missense mutation on the biosynthesis of myeloperoxidase. Materials List 1. / Preparation of extracts and reagents. H.sapiens mRNA for alternatively spliced isoform of CLC-6 chloride channel. Privacy Policy Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. /Encoding /WinAnsiEncoding PubMed: 299761, Pross HF, et al. Contact Us 5 0 obj %PDF-1.4 Cells were treated with 10 ng/ml PMA (EMD Millipore, Billerica, MA, USA) to induce MK differentiation. /Flags 34 Cancer Genet. Res. Organism Type: Human: Erythroleukemia cell line: Tissues: Bone: Phenotype: Suspension: Primary: no: Application: Cell Culture / Growth Conditions,Protein Expression,Protein Purification,Stable Cell Transfection,Transfection: Gene Expression,Transient Transfection Every Step of the Way, a Wide Range of Cell Health Products. /XHeight -546 After two weeks PBMC co-cultured with K562-41BBL-mIL15 were enriched for NK cells (93%) and contained few T cells (0.8%) compared to PBMC cultured with IL2 alone (NK cells 11.9% and T cells 58.7% respectively). /Subtype /TrueType The strain obtained by the ATCC most closely resembles the B population. << /TT2 8 0 R Variation amongst K562 cell cultures. Established from pleural effusion of 53 year old female with chronic myelogenous leukaemia in terminal blast crisis.

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